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(Solved): You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the ...



4. You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in

You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in the old-fashioned way. You set up four reactions, each of which contains the DNA to be sequenced (template), a radiolabeled primer (radiolabel is indicated by *) that can hybridize to the template, DNA polymerase, and the deoxynucleotides (dNTPs) and dideoxy nucleotide (ddNTP) indicated below.
The picture below shows the results when reaction A was run on a gel and subjected to autoradiography. Draw in the results of the other reactions.

The answer is provided below but I would like an explanation on how these answers were determined. Thanks ! 

4. You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in the old-fashioned way. You set up four reactions, each of which contains the DNA to be sequenced (template), a radiolabeled primer (radiolabel is indicated by \( * \) ) that can hybridize to the template, DNA polymerase, and the deoxynucleotides (dNTPs) and dideoxy nucleotide (ddNTP) indicated below. Reactions contain the following nucleotides: A. dATP, dTTP, dCTP, dGTP, ddTTP (results shown below) B. dATP, dTTP, dCTP, dGTP, ddATP C. dTTP, dGTP, dCTP, ddCTP, ddATP D. dATP, dCTP, dTTP, ddGTP The picture below shows the results when reaction \( A \) was run on a gel and subjected to autoradiographv. Draw in the results of the other reactions.


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This method uses the dideoxy sequencing method or end termination method with fluorescent labeling. Where DNA replication is end with the incorporation of modified nucleotides, dideoxynucleotides (ddNTPs). After amplification of the DNA fragment in a
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