
You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in the old-fashioned way. You set up four reactions, each of which contains the DNA to be sequenced (template), a radiolabeled primer (radiolabel is indicated by *) that can hybridize to the template, DNA polymerase, and the deoxynucleotides (dNTPs) and dideoxy nucleotide (ddNTP) indicated below.
The picture below shows the results when reaction A was run on a gel and subjected to autoradiography. Draw in the results of the other reactions.
The answer is provided below but I would like an explanation on how these answers were determined. Thanks !

4. You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in the old-fashioned way. You set up four reactions, each of which contains the DNA to be sequenced (template), a radiolabeled primer (radiolabel is indicated by \( * \) ) that can hybridize to the template, DNA polymerase, and the deoxynucleotides (dNTPs) and dideoxy nucleotide (ddNTP) indicated below. Reactions contain the following nucleotides: A. dATP, dTTP, dCTP, dGTP, ddTTP (results shown below) B. dATP, dTTP, dCTP, dGTP, ddATP C. dTTP, dGTP, dCTP, ddCTP, ddATP D. dATP, dCTP, dTTP, ddGTP The picture below shows the results when reaction \( A \) was run on a gel and subjected to autoradiographv. Draw in the results of the other reactions.