(Solved): Use the diagram above as a reference for the process of amplifying DNA via PCR. You have identifie ...

Use the diagram above as a reference for the process of amplifying DNA via PCR. You have identified a segment of DNA you want to amplify for further analysis via PCR. Answer the related questions below. a. How many unique primers do you need? b. Are your primers part of the sequence you want to amplify, or outside of your region of interest? You need to program the PCR instrument for amplification. Each step (1-3) has a temperature (or range of temperatures) to perform the functions listed (denaturation, annealing, elongation). c. Which step/temperature depends on the primer sequences you are using? d. Which step depends on the optimal polymerase activity temperature? e. Which step has the highest temperature? f. A PCR reaction needs raw materials to make new DNA sequences. List 4 main ingredients in your \( \mathrm{PCR} \) reaction.