(Solved): need help with question 2,4,5 Bacterium for the spore stain is: Bacillus subtilis. Bacteria for cap ...

Bacterium for the spore stain is: Bacillus subtilis. Bacteria for capsule/negative stain are Enterobucter actogenes \& $$\$$$ aureus, Q1. What is the function of a bacterial endospore? Hint: How easy is an endospore to destroy? Use your texibook/ecture notes to help you. Q2. Why does this exercise call for use of an older culture of Bacillus? What does a positive result for a spore stain indicate about the organism? What does a negative result for the spore stain indicate about the microorganism? Q3. List three specific functions of capsules. Use your textbook/ecture notes to help you. (Hint: two of these functions are often listed as functions of various virulence factors). Note: It is not enough to say that it protects bacteria from the immune system or that it is a virulence factor: Q4. In the capsule stain process used in class (using nigrosin), are the bacterial cells heat-fixed to the slide? Why or why not? Q5. Eosin is an acidic red stain and methylene blue is a basic blue dye. What should be the result of staining a smear of encapsulated bacteria with a mixture of eosin and methylene blue? Please note if the capsule is stained or not stained at all.