(Solved): In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only cont ...

In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only contained antibiotics but also inhibitors of other processes such as cholesterol metabolism. Over a period of several years, he screened more than 6,000 fungal cultures until a positive result emerged. This compound, which was named compactin, came from the blue-green mold Penicillium citrinum Pen-5 that was isolated from a rice sample collected at a grain shop in Kyoto. Based on biochemical assays, compactin is the first known statin. 1. The original kinetics data for compactin inhibition of HMG-CoA reductase enzyme that was published by Dr. Endo in 1976 is shown on the right. a) Determine the \( \mathrm{K}_{\mathrm{m}} \) and \( \mathrm{V}_{\max } \) values for enzymatic activity. Do NOI forget the units. Answers can be expressed either as a fraction or in decimals. With No inhibitor: \( \mathrm{K}_{\mathrm{m}}= \) \( \mathrm{V}_{\max }= \) With \( 24 \mathrm{nM} \) compactin: