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(Solved): I need help graphing a ferguson plot of (Log of RF on Y-axis and % gel on X-axis)!! This deals with ...



I need help graphing a ferguson plot of (Log of RF on Y-axis and % gel on X-axis)!! This deals with SDS-page data, finding the RF and comparing them against different % acrylamide gel of 7.5, 10 and 12%. I have the data below with Rf and Rf values below in log, along with the % gel for each on a google sheet!

(yellow column for 7.5%, green column for 10% and purple for 12% gel!) 

I don't get how to do the graph in getting the X-axis for gel % and log rf so it can look like the instructions below in part D. Please help show me how to do this or how to set up cells!! 

File Edit View Insert Format Data Tools Extensions Help Last edit was[A] Your orel. Indicate \( \% \)
[B] How to calculate \( \mathrm{Rf} \) values of standards?
- Calculate the Rf values of all

File Edit View Insert Format Data Tools Extensions Help Last edit was [A] Your orel. Indicate \( \% \) [B] How to calculate \( \mathrm{Rf} \) values of standards? - Calculate the Rf values of all 10 standard proteins in your gel. - Make table as shown below. - Calculation of Rf value for a standard protein, \( 250 \mathrm{kDa} \) is shown below as an example. - Rf value \( =\frac{\text { the distance migrated by each standard protein lane } 1 \text { (a) }}{\text { distance migrated by the solvent dye front (b) }} \) [D] How to draw a Ferguson plot? - Use any three standard proteins, preferably far apart. - Calculate Rf values and find the \( \log \) of them. - Plot Log of Rf on \( Y- \) axis and \( \% \) of gel on X-axis. - Draw trendlines (average). - You should see lines starting at one point on Y-axis and diverging as [C] Prepare a table with Rf values of standards?


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The graph will be drawn in the four quadrants. Where the y axis is n
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