(Solved): Biochemistry question 1. The e. coli chromosome contains 4,641,652 bp. a) How many turns of the he ...

Biochemistry question

1. The e. coli chromosome contains 4,641,652 bp. a) How many turns of the helix need to be unwound during replication? b) If replication occurs at a rate of 1,000 bp/s. If two replication forks proceed from the origin, how long would it take to copy e. coli's genome in minutes? 1 c) About how many Okazaki fragments would be formed during the replication of the e. coli genome? 2. Shown below is a fragment of double stranded DNA. 5'-TCG GTA GCG ATT AGC-3' (coding strand) 3'-AGC CAT CGC TAA TCG-5' (template strand) a) what would be the sequence of the mRNA produced by transcription of the DNA above? Be sure to label the 5' and 3' ends. b) what would be the polypeptide sequence that would result from translation of the mRNA? 3. Below is a piece of mRNA. What point mutations (if any) could be made to this mRNA that WOULDN'T change the final polypeptide sequence? 5'-AUGAUAUUGCUAUCUUGGACU 3'