Below is the titration curve for histidine. The pKa values ??are 1.8 (COOH), 6.0 (side chain), and 9.3 (NH3?). a) Draw the structure of histidine at each ionization step. b) On the titration curve, identify the points that correspond to the four ionic species. c) Identify the points where the average net charge is 2, 0.5, 0, and 1. d)

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Below is the titration curve for histidine. The pKa values ??are 1.8 (—COOH), 6.0 (side chain), and 9.3 (—NH3?).

a) Draw the structure of histidine at each ionization step.
b) On the titration curve, identify the points that correspond to the four ionic species.
c) Identify the points where the average net charge is ˜2, ˜0.5, 0, and ˜1.
d) Identify the point at which the pH equals the pKa of the side chain.
e) Identify the point that indicates the complete titration of the side chain.
f) Between what pH limits would histidine be a good buffer?

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