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(Solved): are my answers correct? Q.3. The scientist wanted to study the mechanism of gene regulation i ...
are my answers correct?
Q.3. The scientist wanted to study the mechanism of gene regulation in lac operon. They identified a mutation in a E. coli cell having the genotype lact lacZ lac \( \mathrm{C}^{+} \)lacA. The scientists cultured the bacteria in presence and absence of lactose and used X-Gal to assay the function of lacZ gene. I) What is the expected level of expression (color assay) of the Beta galactosidase in presence of lactose? a. Beta-Galactosidase will be expressed b. Beta-Galactosidase won't be expressed c. Beta-Galactosidase will be expressed depending on whether glucose is in the c. Beta-Galactosi or not d. None of the above II) What is the expected level of expression (color assay) of the Beta galactosidase in absence of lactose? \( 2 \mathrm{pt} \) a. Beta-Galactosidase will be expressed b. Beta-Galactosidase won't be expressed c. Beta-Galactosidase will be expressed depending on whether glucose is in the media or not d. None of the above