(Solved): A kinetic study of an intestinal peptidase using glycylglycine as the substrate produced the exper ...

A kinetic study of an intestinal peptidase using glycylglycine as the substrate produced the experimental data shown in the table. glycylglycine \( +\mathrm{H}_{2} \mathrm{O} \longrightarrow 2 \) glycine Calculate the standard error of regression (SER) for the Lineweaver-Burk slope and the Eadie-Hofstee slope to compare the precision of each. Lineweaver-Burk SER: \( \mu \mathrm{mol} \mathrm{min}-1 \) Eadic-Hofstee SER: There are several ways to transform the Michaelis-Menten equation so as to plot data and derive kinetic parameters, each with different advantages depending on the data set being analyzed. One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by \( V_{\max } \) and rearranging gives the Eadic-Hofstee equation. \[ V_{0}=-\left(K_{\mathrm{m}}\right) \frac{V_{0}}{[\mathrm{~S}]}+V_{\max } \] Consider the plot of \( V_{0} \) versus \( \frac{V_{0}}{[S]} \) for an enzyme-catalyzed reaction. The control reactions (blue curve) did not contain any inhibitor. Which of the other curves \( (\mathrm{A}, \mathrm{B} \), or \( \mathrm{C}) \) depicts this enzyme's activity in the presence of a competitive inhibitor? curve \( \mathrm{B} \) curve A curve \( \mathrm{C} \) A kinctic study of an intestinal peptidase using glycylglycine as the substrate produced the experimental data shown in the table. \[ \text { glycylglycine }+\mathrm{H}_{2} \mathrm{O} \longrightarrow 2 \text { glycine } \] kinetic study of an intestinal peptidase using glycylglycine as the substrate produced the experimental data shown in the table. \[ \text { glycylglycine }+\mathrm{H}_{2} \mathrm{O} \longrightarrow 2 \text { glycine } \] Calculate the standard error of regression (SER) for the Lineweaver-Burk slope and the Eadie-Hofstee slope to compare the precision of each.