(Solved): 2) What is produced when BAPNA is added to Trypsin? 3) What happens when TLCK is added to Trypsin? ...
2) What is produced when BAPNA is added to Trypsin? 3) What happens when TLCK is added to Trypsin? 4) Describe the transmission of tube 3 when BAPNA and TLCK were added together. Which control tube does it more resemble? 5) Which substrate had the greater affinity for the enzyme trypsin? (Hint: it's the one that wouldn't let the other one bind.) Use tube 3 to make this determination. 6) We used a 1% trypsin concentration. How much trypsin would we need to use to make 1L of a 1% trypsin solution? 7) I had a 1000mg/L concentration stock of BAPNA, and I needed a 1:100 dilution. I had to make 500mL of the substrate. Give instructions for how you would make this solution.