(Solved): 1. We have 2 liters of a \( 3 \mathrm{M} \) solution of Tris-HCl. We need to make \( 500 \mathrm{~m ...

1. We have 2 liters of a \( 3 \mathrm{M} \) solution of Tris-HCl. We need to make \( 500 \mathrm{~mL} \) of \( 300 \mathrm{mM} \) solution. We also have solid componds of Tris-HCl. The molecular weight of Tris-HCl is \( 157.60 \) (6 points) a) What is the starting concentration (C1)? b) What is the final concentration \( (\mathrm{C} 2) \) ? c) What is the starting volume (V1)? d) What is the final volume (V2)? e) What information presented above is not necessary for your calculation? f) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using our starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work. 2. We have \( 200 \mathrm{~mL} \) of \( 8 \% \) SDS. We need to make \( 200 \mathrm{~mL} \) at a concentration of \( 0.5 \% \) solution. The molecular weight of SDS is \( 288.378 \) (7 points) a) What is the starting concentration? b) What is the final concentration? C) What is the starting volume? d) What is the final volume? e) Calculate the dilution factor. Show your work. f) What two pieces of information presented above is not necessary for your calculation? g) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using are starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work. 3. We made \( 450 \mathrm{~mL} \) of \( 2.5 \% \) Luria Broth (LB) for our bacterial cultures, ( 3 points) a) What is the final concentration? b) What is the final volume? c) How many grams of LB powder was used to make the LB? Write the equation used to solved this 4. We need to make \( 8 \mathrm{~mL} \) of \( 20 \% \) methanol solution to dissolve our drug. We already have an \( 80 \% \) solution of methanol. (5 points) a) What is the stock concentration? b) What is the final concentration? c) What is the starting volume? d) What is the final volume? e) Write out the equation used to solve this. Solve the equation and show your work.