(Solved): 1. CHEMOSTAT CULTURE. Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter ...

1. CHEMOSTAT CULTURE. Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. They yield of biomass from substrate is \( 0.06 \mathrm{~g} / \mathrm{g} \), \( Y_{P X} \) is \( 7.7 \mathrm{~g} / \mathrm{g} \). The maintenance coefficient is \( 2.2 \mathrm{~g} /\left(\mathrm{g}^{*} \mathrm{~h}\right) \), the specific rate of product formation due to maintenance is \( 1.1 \mathrm{~h}^{-1} \). The maximum specific growth rate of \( Z \). mobilis is approximately \( 0.3 \mathrm{~h}^{-1} \). The bacteria are cultured in chemostat in a \( 60-\mathrm{m}^{3} \) fermenter. The feed contains \( 12 \mathrm{~g} / \mathrm{L} \) of lucose; \( \mathrm{K}_{\mathrm{s}} \) for the orgnanism is \( 0.2 \mathrm{~g} / \mathrm{L} \). 1) What flow rate Is required for a steady-state substrate concentration of \( 1.35 \mathrm{~g} / \mathrm{L} \) ? 2) At the flow rate of 1 ), what Is the cell density? 3) At the flow rate of 1), what concentration of ethanol is produced?